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| Brissard, Charline; Herve, Fabienne; Sibat, Manoella; Sechet, Veronique; Hess, Philipp; Amzil, Zouher; Herrenknecht, Christine. |
| The presence of Ostreopsis cf. ovata on the Mediterranean coast represents a serious concern to human health due to production of toxins–putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d, -e, -f and -g). However, purified ovatoxins are not widely available and their toxicities are still unknown. In the present study, we report on HR LC-MS/MS analysis of a French Ostreopsis cf. ovata strain (IFR-OST-0.3 V) collected at Villefranche-sur-Mer (France) during a bloom in 2011. Investigation of this strain of Ostreopsis cf. ovata cultivated in our laboratory by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) confirmed the production of ovatoxins -a to–e and revealed the presence of a new ovatoxin... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxins; U-HPLC/HR-MSn; Chromatographic separation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00252/36302/34831.pdf |
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| Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher. |
| Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf |
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| Couet, Douglas; Pringault, Olivier; Bancon-montigny, Chrystelle; Briant, Nicolas; Poulichet, Francoise Elbaz; Delpoux, Sophie; Yahia, Ons Kefi-daly; Hela, Bengharbia; Charaf, M'Rabet; Herve, Fabienne; Rovillon, Georges-augustin; Amzil, Zouher; Laabir, Mohamed. |
| Controlled laboratory experiments were conducted to test the effects of copper (Cu2+) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu2+ (10−4 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC50 values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu2+ for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC50 was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella; Ostreopsis cf. ovata; Cu; Butyltin; Photosynthesis; Toxins. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00418/52970/53896.pdf |
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| Amzil, Zouher; Sibat, Manoella; Chomerat, Nicolas; Grossel, Hubert; Marco-miralles, Francoise; Lemee, Rodolphe; Nezan, Elisabeth; Sechet, Veronique. |
| Dinoflagellates of the genus Ostreopsis are known to cause (often fatal) food poisoning in tropical coastal areas following the accumulation of palytoxin (PLTX) and/or its analogues (PLTX group) in crabs, sea urchins or fish. Ostreopsis spp. occurrence is presently increasing in the northern to north western Mediterranean Sea (Italy, Spain, Greece and France), probably in response to climate change. In France, Ostreopsis. cf. ovata has been associated with toxic events during summer 2006, at Morgiret, off the coast of Marseille, and a specific monitoring has been designed and implemented since 2007. Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a)... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxin-a; LC-MS/MS; Seafood. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18230/15799.pdf |
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| Ben-gharbia, Hela; Yahia, Ons Kefi-daly; Amzil, Zouher; Chomerat, Nicolas; Abadie, Eric; Masseret, Estelle; Sibat, Manoella; Triki, Habiba Zmerli; Nouri, Habiba; Laabir, Mohamed. |
| armful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species. Maximum growth rates were 0.59 ± 0.08 d−1 for O. cf. ovata, 0.35 ± 0.01 d−1 for C. monotis and 0.33 ± 0.04 d−1 for P. lima. Toxin analyses revealed the presence of ovatoxin-a and ovatoxin-b in O. cf. ovata cells. Okadaic acid and... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Prorocentrum lima; Coolia monotis; Southern Mediterranean Sea; Growth; Toxicity. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00355/46585/46393.pdf |
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| Gémin, Marin-pierre; Réveillon, Damien; Hervé, Fabienne; Pavaux, Anne-sophie; Tharaud, Maxime; Séchet, Veronique; Bertrand, Samuel; Lemée, Rodolphe; Amzil, Zouher. |
| Over the last fifteen years, blooms of the genus Ostreopsis have been reported more frequently and at higher abundances in the Mediterranean area. Ostreopsis cf. ovata is known to produce ovatoxins (OVTXs), structural analogues of palytoxin, which is one of the most potent non-polymeric toxins. However, the production of OVTXs is poorly characterized in situ. The present study focuses on toxin content and profile according to the bloom phase during summer 2017 in Villefranche-sur-Mer, France (NW Mediterranean Sea), depth (from 0.5 to 5 m) and three different macroalgal substrates of this epiphytic dinoflagellate (Padina pavonica, Dictyota spp. and Halopteris scoparia). Ovatoxin quantification of all samples was performed by liquid chromatography coupled to... |
| Tipo: Text |
Palavras-chave: Dinoflagellate; Benthic HABs; Ostreopsis cf. ovata; Ovatoxins; Mediterranean Sea. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72566/74835.pdf |
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